Customized Reverse Transcription Service
Trust us to handle your Reverse Transcription needs with precision and care!
A5 DNA Lab offers a highly specialized customized reverse transcription service, designed to convert RNA into complementary DNA (cDNA) with unparalleled efficiency and accuracy. Our experts tailor our reverse transcription protocols to meet the unique requirements of each project, ensuring optimal cDNA yield and specificity. We employ advanced enzymes and cutting-edge technologies to overcome challenging RNA samples, such as limited quantities, degraded RNA, or high GC content. Our customized service includes careful RNA quality assessment, optimized primer design, and rigorous quality control measures to ensure superior cDNA quality. Whether it’s for gene expression analysis, RNA sequencing, or other applications, our customized reverse transcription service delivers high-quality cDNA, empowering researchers to uncover new insights into gene expression, disease mechanisms, and biomarker discovery. At A5 DNA Lab, we prioritize customers’ satisfaction, delivering exceptional results and personalized support to drive innovation in life science research.
PRICE
10 $ (2500 PKR)
note
We will charge only 1 $ (200 PKR) per sample, given that we are provided with cDNA Synthesis Kit by the client.
Frequently Asked Questions
The enzyme reverse transcriptase catalyzes the reverse transcription reaction which is an RNA-dependent DNA polymerase. This enzyme is responsible for transcribing DNA from single-stranded RNA.
Reverse transcriptase is central to the infectious nature of retroviruses, several of which cause disease in humans, including human immunodeficiency virus (HIV), which causes acquired immunodeficiency syndrome (AIDS), and human T-cell lymphotrophic virus I (HTLV-I), which causes leukemia.
Common applications of RT-PCR include detection of expressed genes, examination of transcript variants, and generation of cDNA templates for cloning and sequencing.
In two-step RT-PCR, 3 types of primers, and mixtures thereof, can be used for reverse transcription: oligo-dT primers (typically 13–18mers), random oligomers (such as hexamers, octamers, or nonamers), or gene-specific primers.
