Customized PCR Amplification Service

Trust us to handle your PCR amplification needs with precision and care!

A5 DNA Lab offers a highly specialized customized PCR (Polymerase Chain Reaction) and real-time PCR amplification service, designed to amplify specific DNA and cDNA sequences with precision and sensitivity. Our experts tailor our PCR protocols to meet the unique requirements of each project, ensuring optimal primer specificity, efficient amplification, and accurate quantification. We employ advanced thermocyclers and cutting-edge chemistries to overcome challenging templates, such as low-abundance targets, complex genomes, or degraded DNA. Our customized service includes careful primer design, optimization of PCR conditions, and rigorous quality control measures to ensure superior amplification efficiency and specificity. Whether it’s for gene expression analysis, genetic variation detection, or precise quantification of DNA targets, our customized PCR and real-time PCR service delivers reliable and accurate results, empowering researchers to uncover new insights into gene function, disease mechanisms, and biomarker discovery. At A5 DNA Lab, we prioritize customers’ satisfaction, delivering exceptional results and personalized support to drive innovation in life science research.

PRICE

Varies between 2 $ (500 PKR) to 10 $ (2500 PKR) per reaction, depending on type of the amplification.

note

We will charge only 1 $ (200 PKR) per reaction, given that we are provided with PCR Amplification Kit by the client.

Frequently Asked Questions

Many of the common problems with PCR and RT-PCR are identified during agarose gel electrophoresis of the reaction products. These include the absence of the expected amplification product, the presence of nonspecific products, excessive smearing, and the presence of a “primer dimer” band.

Amplification reagents should be kept in a freezer (or refrigerator, as per manufacturer recommendations) in the same designated space, ideally next to the laminar flow cabinet or pre-PCR area. Gloves should be changed each time upon entering the pre-PCR area or laminar flow cabinet.

If your PCR was successful in making billions of copies of the target segment, you’ll be able to visualize this as a distinct band on the gel. Comparing your PCR samples to control samples (tubes not subjected to PCR) will confirm the success of PCR. Your PCR samples and control samples will be run alongside a DNA ladder.

 

The key ingredients of a PCR reaction are Taq polymerase, primers, template DNA, and nucleotides (DNA building blocks).